Coding
Part:BBa_K4348057:Design
Designed by: Jonathan Cheng Group: iGEM22_McGill (2022-09-25)
SUMO-his
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 281
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 281
Illegal NheI site found at 64 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 281
Illegal BglII site found at 170
Illegal BamHI site found at 361 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 281
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 281
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Contains an EcoRI site, so standard biobrick assembly is not possible. Golden gate or gibson assembly are recommended, or the use of other restriction enzymes.
Source
Saccharomyces cerevisiae